You were tasked to analyze the oxidation rate of glucose by the EMP glycolysis pathway in vitro (in a test tube). You purified all 10 enzymes in the EMP pathway and supplemented glucose, NAD+, and ADP in the test tube. When you analyzed the product pyruvate over a period of time, you found that its concentration was increasing but quickly reached plateau. In order to monitor the rate continuously, you added more glucose in the test tube but found no increase in pyruvate. Your advisor suggested adding two more enzymes in the test tube, a pyruvate decarboxylase (PDC) and an alcohol dehydrogenase (ADH), along with glucose. You were happy to find out that glucose can be continuously oxidized now as long as you supply glucose. 1. What could be the reason that pyruvate stopped increasing in the beginning even with the addition of glucose? (5 pts) 2. What product would you expect to detect when PDC and ADH were added to the test tube? (5 pts) 3. How did the addition of PDC and ADH help with the continuous oxidation of glucose? (5 pts)
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27. When the interest rate on a bond is above the equilibri…
27. When the interest rate on a bond is above the equilibrium interest rate, there is excess ________ in the bond market and the bond price will ________.
What analgesic was developed in 1904 and replaced the use of…
What analgesic was developed in 1904 and replaced the use of cocaine as an analgesic for dental procedures?
A person who holds a DMD is considered a legally certified d…
A person who holds a DMD is considered a legally certified dental hygienist.
A pediodontist deals with the diagnosis and treatment of dis…
A pediodontist deals with the diagnosis and treatment of diseases of the supporting and surrounding structures of the tooth.
Which member of the dental team can diagnose oral disease?
Which member of the dental team can diagnose oral disease?
Which of these advances in dental techniques occurred first?
Which of these advances in dental techniques occurred first?
An endodontist treats pathologies of the dental pulp and sur…
An endodontist treats pathologies of the dental pulp and surrounding tissues.
Dental forensics was first used in 1889 to identify a victim…
Dental forensics was first used in 1889 to identify a victim of a factory fire.
Who is the father of medicine?
Who is the father of medicine?