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Describe the key steps in a western blot experiment (6 points). When comparing relative quantitative difference of a target protein between two samples, scientists often include a western blot on a housekeeping protein, why? (2 protein).

List the three components of a mass spectrometry and their functions.

The expectation that exercise can function to reduce pain and/or increase pleasure is linked to which hypothesis?

Anxiety is a relatively complex mental health condition with many characteristics. Class lecture provided which of the following descriptions of anxiety?  This is a multiple answer question. At least one response is correct.  Select all that are correct. 

Clinical anxiety symptoms could be accurately described as: 

In single-cell RNAseq analysis, what is the use of Unique Molecular Identifier (UMI)?  

In Western blot, indirect detection using an unlabeled primary antibody followed by a labelled secondary antibodies increases the detection sensitivity.

To study gene expression, neither microarray nor highthroughput sequencing requires a priori knowledge of the gene sequences, only that highthroughput sequencing gives more data.

What is the role of Protospacer Adjacent Motif (PAM) in the CRISPR/Cas9 system?

The next two questions are related to the following issue. Mr A presents with a sore red tongue, fatigue and difficulty concentrating. A blood test is requested to investigate the cause of the patient’s symptoms.