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  Show all supporting work. If needed, round answers to the nearest tenth. ZY = [27] WY = [38.2] RX = [19.1]

During electrophoresis, DNA moves towards ——— electrode.

In a transformation experiment, the non-transformed cells plated on LB+Ampicillin plate showed growth.  What would this indicate?

In the transformation experiment performed, the GFP gene confers resistance to the antibiotic Ampicillin.

In the following picture, X represents which enzyme?

During transformation of bacteria with pGFP, any bacteria that have not taken up the pGFP plasmid will not grow on which of the following plates?  

You conduct an ELISA sample to test the presence of antibodies in a patient sample.  After conducting the entire experiment, you suddenly realize that you did not add any substrate (antigen) to the wells in the initial step.  Under such circumstances, the development of a colored reaction in the patient sample (positive result) would indicate a ——-

Cells that have NOT undergone transformation with pGFP should be killed after plating  on LB-AMP.  

In the following figure, the 1:32 dilution of the sample will have less antibodies than the 1:2 dilution (assuming that the dilutions are done correctly)

In a transformation experiment, nutrient Agar plates containing the antibiotic Ampicillin were used in the experiment for what purpose?