In a kinetic nephelometric assay in which the slope of the i…

In a kinetic nephelometric assay in which the slope of the increase in light scatter between an initial and a subsequent timepoint is used to determine the concentration of the antigen to be measured, it is important to ensure that increasing antigen concentration is always associated with increasing light scatter (increasing precipitation).  This condition applies throughout which zone(s) of the antigen-antibody precipitation curve:

In a kinetic nephelometric assay in which the slope of the i…

In a kinetic nephelometric assay in which the slope of the increase in light scatter between an initial and a subsequent timepoint is used to determine the concentration of the antigen to be measured, it is important to ensure that increasing antigen concentration is always associated with increasing light scatter (increasing precipitation).  This condition applies throughout which zone(s) of the antigen-antibody precipitation curve: