Materials: Three clean test tubes Four beakers Two 5 mL Graduated pipet Hydrogen peroxide (H2O2) Catalase (potato) Ruler Wax pencil Procedure: In order to test enzyme concentration, we will be diluting the catalase with water. Use the beakers to dilute the catalase. Mix according to the table below. Beaker Water Catalase Concentration 1 3 mL of water 1 mL of Catalase 25% 2 2 mL of water 2 mL of Catalase 50% 3 1 mL of water 3 mL of Catalase 75% 4 0 mL of water 4 mL of Catalase 100% Label each of your test tubes (E1, E2, E3, and E4). To each of your test tubes add 4 mL of hydrogen peroxide. To E1, add 1 mL of 25% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E2, add 1 mL of 50% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E3, add 1 mL of 75% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E4, add 1 mL of 100% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. Results: (Watch the Lab 7. Enzymes video) Table 3: Effects of Enzyme Concentration (Copy and paste the table to answer Question 18 and fill it in with your data) Test Tube Catalase Concentration Bubble Height (mm) E1 E2 E3 E4
Blog
Which condition is mostly likely to cause V/Q mismatch?
Which condition is mostly likely to cause V/Q mismatch?
What is the primary physiological effect of high-flow oxygen…
What is the primary physiological effect of high-flow oxygen therapy?
Which of the following is NOT a group we usually associate w…
Which of the following is NOT a group we usually associate with being at risk for malaria infection?
Which of the following drugs is a long-acting bronchodilator…
Which of the following drugs is a long-acting bronchodilator?
Which of the following statements is TRUE concerning the dif…
Which of the following statements is TRUE concerning the difference between sterile water and distilled water? I) Sterile water contains additives with bacteriostatic properties. II) Sterile water is an isotonic solution. III) Distilled water is an isotonic solution. IV) Distilled water is considered to be both sterile and pure.
Which of the following is considered the most important pred…
Which of the following is considered the most important predisposing factor to airway irritation and mucus hypersecretion with chronic bronchitis?
Which of the following effects does histamine have on system…
Which of the following effects does histamine have on systemic circulation?
A parasympathomimetic agent that intensifies the level of br…
A parasympathomimetic agent that intensifies the level of bronchial tome to the point of bronchial constriction and is used in bronchial provocation testing is:
Which of the following are therapeutic options for controlli…
Which of the following are therapeutic options for controlling mucus hypersecretion, other than using mucoactive agents? I. Remove causative factors, such as pollution, where possible II. Treat infections III. Optimize tracheobronchial clearance, including the use of bronchodilators IV. Incorporate bronchial hygiene measures, such as cough and postural drainage