Part 4: Effect of pH in Enzyme Activity  Each enzyme has an…

Part 4: Effect of pH in Enzyme Activity  Each enzyme has an optimal pH that helps maintain its three-dimensional shape. Changes in pH may denature enzymes by altering the enzyme’s charge. This alters the ionic bonds of the enzyme that contribute to its functional shape. 

Materials:  Three clean test tubes  Test tube rack  Five 5…

Materials:  Three clean test tubes  Test tube rack  Five 5 mL Graduated pipet   Hydrogen peroxide  Catalase (potato)  Water pH 3  Water pH 7  Water pH 11  Ruler  Wax pencil  Procedure:  Label each of your test tubes (P1, P2, and P3).   To P1, add 1 mL of Catalase and 2 mL of water pH 3.  To P2, add 1 mL of Catalase and 2 mL of water pH 7.  To P3, add 1 mL of Catalase and 2 mL of water pH 11.  Wait 1 minute before proceeding.  Add 4 mL of hydrogen peroxide to all three test tubes.   Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 4.  Results: (Watch the Lab 7. Enzymes video)  Table 4: Effects of pH in Enzyme Activity (Copy and paste the table to answer Question 27 and fill it in with your data) Test Tube  pH  Bubble Height (mm)  P1      P2      P3     

Materials:  Two clean test tubes  Test tube rack  Ruler  1…

Materials:  Two clean test tubes  Test tube rack  Ruler  1 mL graduated pipet  Catalase (from potato)  Hydrogen Peroxide  Distilled water (dH2O)  Wax Pencil  Procedure:  Obtain two test tubes and label one as A and one as B.  Fill each of two test tubes with 1 mL of catalase (from the potato).  Add 10 drops of hydrogen peroxide to the tube marked A.  Add 10 drops of distilled water to tube marked B.  Wait 60 seconds and measure the height of any bubbling you observe, record your results in Table 1.  Results: You will need to watch the Lab 7. Enzymes video to answer the questions. https://youtube.com/embed/8ljGQuvXRZo Table 1: Catalase Activity (Copy and paste the table to answer Question 3 and fill it in with your data) Test Tube  Contents  Bubble Height (mm)  1  Catalase Hydrogen Peroxide    2  Catalase Water