T-cell receptor gene rearrangements were analyzed by PCR usi…

T-cell receptor gene rearrangements were analyzed by PCR using forward and reverse primers complementary to the Vg1 and Jg1 regions, respectively, of the T-cell receptor gene region. The products were separated by polyacrylamide gel electrophoresis and bands visualized using ethidium bromide. The lane corresponding to the patient had a sharp band. All controls were acceptable. How is this result interpreted?

A patient is assessed for B-cell clonality by examining the…

A patient is assessed for B-cell clonality by examining the immunoglobulin heavy chain gene rearrangements using PCR with primers complementary to the variable and joining regions of the immunoglobulin heavy chain gene. When the products are resolved by gel electrophoresis, the patient lane has a smear and does not have a sharp band. Controls were all acceptable. This result is interpreted as which of the following?