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T-cell receptor gene rearrangements were analyzed by PCR using forward and reverse primers complementary to the Vg1 and Jg1 regions, respectively, of the T-cell receptor gene region. The products were separated by polyacrylamide gel electrophoresis and bands visualized using ethidium bromide. The lane corresponding to the patient had a sharp band. All controls were acceptable. How is this result interpreted?

A patient is assessed for B-cell clonality by examining the immunoglobulin heavy chain gene rearrangements using PCR with primers complementary to the variable and joining regions of the immunoglobulin heavy chain gene. When the products are resolved by gel electrophoresis, the patient lane has a smear and does not have a sharp band. Controls were all acceptable. This result is interpreted as which of the following?

Which are used in crossmatching for organ transplant?

Gene expression patterns are now used to characterize tumor types. What method is appropriate for analysis of expression of many genes simultaneously?

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

Which of the following describes a gene rearrangement? 

Mutation in Myeloproliferative leukemia (MPL) gene is found in which of polycythemia vera.

What is the relationship between the number of HLA alleles that have been identified by serological methods as compared with DNA analysis methods?

A set of alleles on the same chromosome is called which of the following?

HLA specificities were first defined in the laboratory based on which type of methodology?