You need to make 100mL of TE pH 8.0 buffer (consisting of 12…

You need to make 100mL of TE pH 8.0 buffer (consisting of 120mM Tris and 1mM EDTA) for a DNA extraction. You have Tris base powder and EDTA powder from which to make it. Tris powder has a MW of 121g/mol, while EDTA powder has a MW of 292g/ml. [Answer 1] How much Tris powder will you need for this desired volume of TE solution? [Answer 2] How much EDTA powder will you need?

What property of trypan blue and similar liquids contributes…

What property of trypan blue and similar liquids contributes to errors or inaccuracies in measurements, especially when pipetting? In other words, what led to your obtaining pipetting CV values greater than 10% during your skills assessment (assuming, of course, you weren’t yet a pipet champ)?