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Conclusion: Answer the following questions for Part 3: Effect of Enzyme Concentration in Enzyme Activity.

Hypothesis: Based on the information provided above come up with a hypothesis for your experiment.

What is the optimum temperature for the catalase to function, explain your answer?  

Materials:  Two clean test tubes  Test tube rack  Ruler  1 mL graduated pipet  Catalase (from potato)  Hydrogen Peroxide  Distilled water (dH2O)  Wax Pencil  Procedure:  Obtain two test tubes and label one as A and one as B.  Fill each of two test tubes with 1 mL of catalase (from the potato).  Add 10 drops of hydrogen peroxide to the tube marked A.  Add 10 drops of distilled water to tube marked B.  Wait 60 seconds and measure the height of any bubbling you observe, record your results in Table 1.  Results: You will need to watch the Lab 7. Enzymes video to answer the questions. https://youtube.com/embed/8ljGQuvXRZo Table 1: Catalase Activity (Copy and paste the table to answer Question 3 and fill it in with your data) Test Tube  Contents  Bubble Height (mm)  1  Catalase Hydrogen Peroxide    2  Catalase Water   

Explain your results for Test tube 2. How did the temperature influence the enzyme activity?

Materials:  Three clean test tubes  Four beakers  Two 5 mL Graduated pipet   Hydrogen peroxide (H2O2)  Catalase (potato)  Ruler  Wax pencil  Procedure:  In order to test enzyme concentration, we will be diluting the catalase with water. Use the beakers to dilute the catalase. Mix according to the table below.  Beaker  Water  Catalase  Concentration  1  3 mL of water  1 mL of Catalase  25%  2  2 mL of water  2 mL of Catalase  50%  3  1 mL of water  3 mL of Catalase  75%  4  0 mL of water  4 mL of Catalase  100%  Label each of your test tubes (E1, E2, E3, and E4).  To each of your test tubes add 4 mL of hydrogen peroxide.   To E1, add 1 mL of 25% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E2, add 1 mL of 50% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E3, add 1 mL of 75% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E4, add 1 mL of 100% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  Results: (Watch the Lab 7. Enzymes video)  Table 3: Effects of Enzyme Concentration (Copy and paste the table to answer Question 18 and fill it in with your data) Test Tube  Catalase Concentration  Bubble Height (mm)  E1      E2      E3      E4     

Which condition is mostly likely to cause V/Q mismatch?

What is the primary physiological effect of high-flow oxygen therapy?

Which of the following is NOT a group we usually associate with being at risk for malaria infection?

Which of the following drugs is a long-acting bronchodilator?