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For each of the following polymerases, state what it uses as a primer. Choose from the terms below. E. coli DNA polymerase III – uses [1] as primer Telomerase – uses [2] as primer Reverse transcriptase – uses [3] as primer   Choose from those terms:  DNA, RNA, tRNA, mRNA You can use one term more than once.  Please watch your spelling!

The following enzymes are actually RNA molecules with catalytic (enzymatic) activity: Endonuclease RNase P (tRNA processing)

What prevents joining of a V region to another V region during VDJ recombination?

Which of the following statements about nucleotides are true and which are false?    In DNA, the bases are connected to the backbone by glycosidic bonds

5’ to 5’ linkages are found in nucleic acids: in tRNAs as a post-transcriptional modification

What is the role of dideoxynucleotides in DNA sequencing?

The artist Heather Dewey-Hagborg (a real person, you can google her after the exam) collected discarded cigarette butts, chewing gum and fingernails left behind on the sidewalks of New York City. She then extracted DNA, had it analyzed for the presence of certain markers that can inform phenotype (e.g. eye color, skin color, nose size) and used that information to reconstruct 3D portraits of the people that left the original items behind. Do you think this kind of project is problematic? In what way? Explain your arguments whether you see a problem or not. Do you think data collected in such a way could be used as a means of surveillance? Should this then be regulated to avoid abuse?

  This DNA sequence occurs within the coding sequence of a gene. The first three nucleotides (underlined) code for glutamic acid (Glu).  The following DNA mutation is likely to result in a shorter than normal mRNA: G -> C at position 70  

Which of the following statements about nucleotides are true and which are false?    In DNA, the sugar is ribose, while in RNA it is deoxyribose

Which of the following statements about antibody molecules are true and which are false?  They form by DNA recombination between D (diverse) and C (constant) regions