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Upload your answers to these questions. Outline the synthesis of the ketone body, acetoacetate, which forms in the liver under fasting conditions. It is formed in three steps. Include all substrate and product names and structures, enzyme names, and any necessary cofactors or coenzymes. (6 pts.) For the last step in the synthesis of acetoacetate, draw the arrow pushing mechanism for the reaction and identify which reaction in glycolysis it is parallel to. (3 pts.)  Ketone bodies are exported from the liver and utilized as an energy source by the extra-hepatic tissues. The brain, which normally utilizes glucose as its energy source, can, under fasting conditions, partially convert to using ketone bodies over the course of a few days. Show how a molecule of the ketone body, -hydroxybutyrate, produced in the liver and exported via the blood to the brain, is utilized for energy production and what advantage this compound has over acetoacetate in terms of cellular energy production. You may use compound names instead of structures. Enzyme names are not necessary. Include any necessary cofactors for full points. (3 pts.) 

Upload your answers to these questions. Beginning with PLP (Vit. B6), whose structure is given below, draw out the arrow-pushing mechanism that results in the transfer of the amino group of Ala to PLP as catalyzed by the enzyme alanine aminotransferase. (6 pts.)  Name the molecule that ultimately accepts the amino group of Ala in the above alanine aminotransferase reaction. (1 pt.)  The α-amino group in the amino acid glutamate can be directly converted to an α-keto compound in a single step with the release of ammonia. This reaction requires a cofactor. Identify the cofactor and the general name for the type of enzyme that catalayzes reactions like these. (2 pts.) 

Write down how much he should be fed per day and per meal, including treat allotments. Be precise, and remember everything I said about communicating with owners.

What is the DMB % of crude fat in his wet food?

Excluding treats, how much does Kiba cost to feed per day?

How many times a day will Kiba be fed if the doctor wants Kiba fed every 4 hours?

You’ve already calculated, to the nearest whole number, how many cups of food he will be fed per day. How many days will the bag of dry dog food last? Round to the nearest whole number.

Approximately how many cups of dry food are in the bag of dog food?

Show me that your instructions above match the daily MER.

The owners want to feed the following foods: Royal Canin Giant Breed Adult. A 30 lb bag costs $99.99. 1 cup of food weighs 108 grams.   Royal Canin Joint Care Loaf in Sauce Canned Dog Food It costs $48.08 for 13.5oz x 12 cans before tax. MilkBone Birthday Cake Artificially Flavored Dog Biscuits