2. Which of the following is the most logical order of a typ…

2. Which of the following is the most logical order of a typical gene cloning experiment?   I – Recombination of gene into cloning vector (sub-cloning) II – Cloning and selection III – Restriction digestion of gene and cloning vector DNA IV – Transformation to host cell V – Isolation of gene and cloning vector DNA through agarose gel electrophoresis

9. Place the steps in a cycle of PCR (Polymerase Chain React…

9. Place the steps in a cycle of PCR (Polymerase Chain Reaction) in the correct order.    1.Annealing—Cool mix to allow primers to form hydrogen bonds with ends of target sequence 2.Extension—DNA polymerase adds free nucleotides to the 3′ end of each primer 3.Denaturation—Heat briefly to near boiling temp to separate DNA strands