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Briefly explain how the quaternary structure of this protein is arranged.     

You have joined the Wee lab as an undergraduate student. This lab’s expertise is in protein engineering. You are tasked with purifying proteins for the research group.  The protein is a DNA polymerase (which binds negatively charged DNA) from a thermophilic organism (Pseudomonas furiosis). Your colleague has cloned this gene in the bacteria E. coli. Because the enzyme’s activity is negatively impacted by the addition of affinity tags, no tag was added to the gene recombinantly expressed.  Your goal is to purify this protein to a high degree of purify (>95%). But, you are challenged to purify your assigned protein in no more than two steps.  In your lab, you have access to the following chromatography techniques and columns.  Cation exchange chromatography Anion exchange chromatography Protein A chromatography Immobilized metal affinity chromatography Amylose resin Sepharose B resin Hydrophobic interaction chromatography Size exclusion chromatography Heat treatment Ammonium sulfate precipitation Your response to this question may take the following format.  For step 1, I will choose method X. Method X separates between based on property A. I chose method X because ….. For step 2, I will choose method Y Method Y separates proteins based on property B. I chose method Y because….  If you used a chromatography column in steps 1 and/or 2, you must specify how you will operate the column to retrieve (elute) your protein (increasing or decreasing salt gradient, pH adjustment, molecule addition). 

Match each description to the corresponding organelle. 

What relative values of KM  and kcat  would describe an enzyme with a high catalytic efficiency?

Which sugar CANNOT be fed into the glycolytic pathway in humans?

The Catholic priest Bartolomé de Las Casas spoke out against

Explain the experience of the African slave and the indentured servant that ultimately ended up in the American colonies int he early colonial period (trace his/her experience beginning in Africa or Britain). Be sure to explain how slavery and servitude was introduced to colonial America, and the economic forces that drove it both in the colonies and abroad.

When globbing, what character(s) is/are used to represent zero or more characters?

Using the exponential smoothing model for forecasting, the smoothing constant alpha determines the level of smoothing and _____________

The nurse is teaching a patient with myasthenia gravis how to recognize a cholinergic crisis. What manifestations should the nurse include in this teaching? (Select all that apply.)