Recent research has shown that some Rho utilization sequence…

Recent research has shown that some Rho utilization sequences (rut sites) can also double as a distinct Nus utilization sequences (nut sites) in some bacterial RNAs (i.e. these sequences overlap substantially). This has led to the hypothesis that NusA, which appears to compete with Rho for RNA binding at these rut+nut elements, may act to inhibit the action of Rho until transcription has appropriately progressed. Select the most logical outcome of each hypothetical perturbation.   Mutating the overlapping rut/nut element such that only Rho can bind will most likely cause [blank1]. Mutating the overlapping rut/nut element such that only NusA can bind will most likely cause [blank2].

You are researching a bacterial transcriptome when you disco…

You are researching a bacterial transcriptome when you discover what appears to be an uncharacterized gene (RNA sequence that aligns to the genome in a place with no known/annotated gene). About 10 bases upstream of this suspected gene, you find a typical sigma (σ) binding site. In order to study the function of this suspected gene, you use targeted mutagenesis to delete the sigma binding site. What is the most likely outcome of this mutation?