Part 4: Effect of pH in Enzyme Activity  Each enzyme has an…

Part 4: Effect of pH in Enzyme Activity  Each enzyme has an optimal pH that helps maintain its three-dimensional shape. Changes in pH may denature enzymes by altering the enzyme’s charge. This alters the ionic bonds of the enzyme that contribute to its functional shape. 

Materials:  Three clean test tubes  Test tube rack  Five 5…

Materials:  Three clean test tubes  Test tube rack  Five 5 mL Graduated pipet   Hydrogen peroxide  Catalase (potato)  Water pH 3  Water pH 7  Water pH 11  Ruler  Wax pencil  Procedure:  Label each of your test tubes (P1, P2, and P3).   To P1, add 1 mL of Catalase and 2 mL of water pH 3.  To P2, add 1 mL of Catalase and 2 mL of water pH 7.  To P3, add 1 mL of Catalase and 2 mL of water pH 11.  Wait 1 minute before proceeding.  Add 4 mL of hydrogen peroxide to all three test tubes.   Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 4.  Results: (Watch the Lab 7. Enzymes video)  Table 4: Effects of pH in Enzyme Activity (Copy and paste the table to answer Question 27 and fill it in with your data) Test Tube  pH  Bubble Height (mm)  P1      P2      P3