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A pathologist requests both the Alcian blue, pH 2.5 and a Periodic Acid Schiff stain to be done on a tissue sample. These stains can be used to differentiate:

Formalin pigment is identified in a section of spleen. This pigmentation can be removed by treating the sections with:

A pathologist suspects that a patient has Alzheimer’s disease, what stain would be helpful in making this determination?

A quality control record indicates the paraffin melting point is between 52 C to 54 C degrees. Ideally the temperature for the tissue flotation bath should be established at:

A section of skin shows an abundant amount of melanin which is obscurring the cellular detail.  Which technique can be used to bleach melanin from tissues?

Microscopic evaluation of a Masson trichrome reveals that the connective tissue is staining a faded blue instead of the expected crisp bright blue color. What can be done to prevent this from happening again?

What most likely caused the reaction shown here of a section of kidney stained with PAS?  10(2).jpg 

The most suitable method of determining the end point of decalcification for a bone specimen fixed in Zenker’s would be….?

The tissue shown here was stained with the GMS stain.  What is the most likely explanation for the finding shown here?  53.jpg 

The demonstration of myelin can be accomplished with the __________ stain.