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You are studying a microRNA, miRNA1, and decide to PCR amplify its entire coding region and add it to the multiple cloning site (MCS) of the plasmid shown below (the intended “insert”). miRNA1 is 22 nucleotides in length. After attempting your cloning reaction, you transform the plasmid into bacteria and grow the bacteria with both X-Gal and IPTG on their growth medium. Select all correct results you should expect when attempting this cloning procedure. -The MCS is a cluster of restriction endonuclease cut sites used to add DNA to the plasmid via restriction-ligation cloning). -“lacZ” indicates a functional lacZ gene with the normal promoter and operator from the lac operon. This gene is functional with a small MCS added to it in-frame (as shown above), but any frameshift OR the addition of >200 bp of DNA within the MCS abolishes lacZ function. -X-Gal is a substrate of the lacZ gene product that turns bacteria from colorless/white to blue when it is metabolized. -IPTG is an allolactose analog that prevents LacI from binding the lac operator. -Ampicillin is a commonly used antibiotic that kills all bacteria that do not express an AmpR resistance gene (which is also found on the plasmid).

Approximately what percentage of the human genome are “housekeeping” genes that are expressed in most cells?

True or false: all species of animals with male and female sexes undergo dosage compensation of a sex chromosome by inactivating one copy of a chromosome in females (usually the X chromosome). 

Below is shown a simplified hypothetical organism, eggman. You are researching gene1, and intend to create a transgenic eggman line that constitutively overexpresses gene1 across all regions of its body throughout development. Given the scheme below, describe the smallest/simplest transgene that could be added to the eggman genome to accomplish expression of gene1 throughout the entire body of eggman. Your answer should interfere with other genes as little as possible, and your answer does not need to name components related to genomic integration methods, only the functional transgene locus that could drive gene1 expression throughout eggman. [Answers should fewer than ~10 words, >20 words results in -0.5] Reminder about how eggman works: four transcription factors (magenta, yellow, green, and blue) are all maternally deposited in the pattern shown in the drawing on the left. Those transcription factors (TFs) bind enhancers corresponding to their color (“E” boxes) and drive expression of the head, arm, and leg genes. In this version of eggman, we also have a black transcription factor that is driven by all four maternally deposited TFs, as well as itself. The black TF also drives the expression of an important housekeeping gene that is required for cell survival in eggman. 

  (Choose all that apply): Which of the following is true about this code?

Which of the following would be the right time to use a button?

What is the Document Outline used for?

What is the value of myNumber at the end of the following code? let a = 6 let b = 4 let c = 2 let myNumber = a+ b * c

(Choose all that apply): What are the three primary goals of Swift

What do you use to switch to running code on a physical device instead of using Simulator?