You plate out a liquid culture of E. coli on TSA containing…

You plate out a liquid culture of E. coli on TSA containing the antibiotic streptomycin, incubate for 24 hours, and observe a single colony. From this you obtain a pure culture of streptomycin resistant E. coli, which you propagate on TSA with streptomycin in the following weeks. You then inoculate your culture onto TSA without streptomycin and continue propagating it over the coming months on TSA lacking the antibiotic. Months later, you discover your E. coli strain is no longer resistant to streptomycin. Please explain this result: why was your original colony resistant to streptomycin, and why, months later, did you end up with a culture that was not resistant to the antibiotic?