The plasmid pAEC-26 (FIGURE 1) was digested with the restric…

The plasmid pAEC-26 (FIGURE 1) was digested with the restriction enzymes below: A) XbaI B) BseRI + XbaI C) BseRI+ PmeI D) XbaI + PmeI After the digestion, all samples were individually loaded in a 1.5% agarose gel and the fragments were separated via gel electrophoresis. In that gel (FIGURE 2) a molecular weight marker was used in the lane 1. The problem is that the mixtures were not loaded in the order above. Can you please indicate the lines in which each of the mixtures were loaded?

The pCrazy45 was used to clone the alpha amylase gene. The p…

The pCrazy45 was used to clone the alpha amylase gene. The plasmid map carrying the alpha amylase encoding the ORF correctly inserted downstream of the T7P is shown in the picture below. Describe the results you expect to have in agar plates having just LB sucrose 5% to recover the Escherichia coli colonies after transformation? The red circle with a line represents the T7 terminator.