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What are the two key components in pUC19 that would allow you to screen for recombinant colonies? Image Long Description A circular diagram labeled “pUC19” with “2686 bps” written in the center. The circle has colored sections: a green section on the left labeled “Amp”, a blue section on the bottom labeled “pMB1 ori”, and a blue section on the top right with “lacZ” labeled. There are small colored rectangles (red, blue, and other colors) positioned along the circle’s edge near the top right, with labels including “MCS”, “LacR binding site”, and “Plac”. On the right side of the circle is a vertical list of text labels including “ApaI”, “EcoRI”, “BamI”, “SacI”, “KpnI”, “AvaI”, “SmaI”, “XmaI”, “BamHI”, “XbaI”, “SalI”, “HincII”, “AccI”, “SspI”, “PstI”, “SphI”, and “HindIII”. Arrows indicate direction around the circular diagram.  

Identify which baby belongs to which set of parents. Image Long Description A rectangular diagram with column headers across the top reading “Couple A”, “Couple B”, “Couple C”, “Baby 1”, “Baby 2”, and “Baby 3”. Under each couple heading are the letters “M” and “F”. The diagram contains horizontal black bars of varying lengths arranged in columns under each heading. At the top of each column are gray outlined rectangles. A vertical dashed line separates the couple columns from the baby columns on the right side. The black bars are scattered throughout the columns at different vertical positions, with some bars appearing longer or shorter than others.    

In her internal monologue, Myrrha reasons: “For all the creatures it is natural / When the bull mounts the heifer, his daughter, / Neither feels shame. / A stallion fights to breed from his own daughter… / Man has distorted that licence / Man has made new laws from his jealousy / To deprive nature of its nature.” What does Myrrha’s argument from animal nature most directly reveal about the structure of her reasoning?

When Diana sees Actaeon, the text describes: “She twisted her breasts away, showing him her back. / Glaring at him over her shoulder / She blushed like a dawn cloud… / And raged for a weapon, for her arrows / To drive through his body. / No weapon was to hand, only water.” What does the substitution of water for arrows most directly reveal about the nature of Diana’s punishment?

When the statue comes to life, Pygmalion reacts in the following way: “He knew / Giddy as he was with longing and prayers / This must be hallucination. / He jerked himself back to his senses / And prodded the ivory. He squeezed it. / But it was no longer ivory. / Her pulse throbbed under his thumb.” What does Pygmalion’s act of prodding and squeezing the statue most directly reveal about his state of mind?

The text explains the origin of Pygmalion’s aversion to living women: “The spectacle of these cursed women sent / Pygmalion the sculptor slightly mad. / He adored woman, but he saw / The wickedness of these particular women / Transform, as by some occult connection, / Every woman’s uterus to a spider.” What does the phrase occult connection most directly establish about the nature of Pygmalion’s phobia?

During the nurse’s interrogation, the text records twice: “As if she had heard nothing else / That single word father went through Myrrha / Like a hot iron, and she sighed in misery. / The nurse missed that clue.” And later: “Again at the word father / Myrrha choked a cry.” What does the repeated triggering of this single word most directly reveal about Myrrha’s condition?

After the assault, Callisto’s behavior within Diana’s company is described: “She no longer led the troop, / Was no longer the boisterous nearest / To the goddess. She hung back, eyes to the ground, / As if slinking along from hiding to hiding.” What does Callisto’s physical withdrawal from the group most directly reveal about the consequences of the assault?

During the festival of Venus, Pygmalion struggles to articulate his prayer: “He hardly dared to think / What he truly wanted / As he formed the words: O Venus, / You gods have power / To give whatever you please. O Venus, / Send me a wife. And let her resemble, / He was afraid / To ask for his ivory woman’s very self. / Let her resemble / The woman I have carved in ivory.” What does Pygmalion’s refusal to name his true wish most directly reveal about his relationship to his own desire?

After the rape, Philomela declares to Tereus: “I may be lost, / You have taken whatever life / I might have had, and thrown it in the sewer, / But I have my voice. / And shame will not stop me. / I shall tell everything / To your own people, yes, to all Thrace.” What does Philomela’s declaration most directly establish about her understanding of her own situation?