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After you completed your exam, scan your solution pages into a single PDF, name it Exam2_Student Name (example: Exam2_JohnSmith).  Before uploading, verify your file has all the pages you need to submit. Upload it by clicking on “Choose a File” button in “Question 1” below. You are only allowed to upload one file to a question. Then click “Submit Quiz”. You may get a warning saying that you have some unanswered questions if you do not use optional file upload sections, click OK to submit anyway. View quiz document here. Use double-sided arrow icon on the tool bar below to preview it in full screen mode. You can also download it by clicking on the downwards arrow to the right of the exam file and open it on your computer. TEST2 (1).pdf

Which enzyme is primarily responsible for adding new deoxyribonucleotides to a growing DNA strand during replication in prokaryotes?

In prokaryotes, in which part of the cell do replication, transcription, and translation occur?

A researcher constructs a plasmid that carries a constitutively active ampicillin resistance gene. They introduce this plasmid into a population of bacterial cells. After transformation, the researcher needs to identify which cells have successfully taken up the plasmid. Question:Which type of growth medium should the researcher use to ensure that only the bacteria containing the plasmid survive, and why?

A researcher has a very small sample of DNA and needs to make millions of copies in order to study a specific gene. Because the original amount of DNA is insufficient for experiments like sequencing or cloning, the researcher decides to use a laboratory technique that can rapidly amplify the DNA. Question:Which technique should the researcher use to amplify the DNA, and what is the basic principle behind it?

Which plasmid carries the genes necessary to facilitate its own conjugation between bacterial cells?

Which of the following best defines genetic engineering?

A researcher is using genomics approaches to identify pathogens in patient samples by analyzing their DNA. While this method works well for most microbes, the researcher notices that certain infectious agents cannot be reliably detected using standard genomics techniques. Question:Which type of pathogen would genomics NOT be effective in detecting?

A ________ blot is used to detect specific DNA sequences on a membrane, while a ________ blot is used to detect specific RNA sequences on a membrane. embrane.

Which of the following enzymes is responsible for joining two sugar-phosphate backbones of DNA?