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In the following question, match the filtercubes (excitation filter, dichroic mirror, emission filter) below to the most appropriate fluorophores. Filtercube 1:   Filtercube 2:   Fluorophore choices mCerulean3   B. mEGFP   C. mOrange2   D. PATagRFP

Which diagram represents the correct concentration gradient if a protein in solution is subjected to AUC, but no diffusion occurs during the centrifugation?

When beads are trapped in a laser beam, the refraction generates a force away from the focal point of the laser.

List two ways in which FRET efficiency can be measured. Briefly describe one of the two ways.

Explain briefly the concept of iTRAQ and how absolute protein concentrations can be measured using this approach.

Fill in the blanks: Phase contrast microscopy converts a difference in [variable1] to a difference in [variable2].

CALORIMETRY

OPTICS

Which force(s) counteract(s) the gravitational force?

Give two approaches that can be implemented to solve the mass transport limitation