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You have been asked to express protein X in E. coli. Upon cloning the gene encoding this protein into your favorite expression vector, you realize that you first of all have a hard time cloning (obtaining only few transformants) and that cells transformed with the plasmid (that you finally managed to make), grow very slowly and cultures never reach high cell densities.  Briefly answer the following two questions: 1. What is the most likely reason for your difficulties? 2. How can you overcome this challenge? Name three strategies that you could use to solve your problem.

Which enzyme class is most widely used for industrial biocatalysis? Name 3 examples for enzymes from this class.

You have been given the task to develop a new biocatalytic process utilizing an esterase as catalyst. Currently no esterase is available that is sufficiently active for the catalytic process you are developing. Briefly describe at least 4 strategies how you would derive/identify a suitable esterase for this process.

   Match the words below with the respective number found the amino acid metabolism scheme above. Oxaloacetate [6] Anaplerotic reactions [16] Tryptophan [13] Pyruvate [2] Shikimate [12] Phenylalanine [14] Lysine [9] Citrate [4] Phophoenolpyruvate [1] Pentose phosphate [11] Acetyl-CoA [3] Aspartic acid [7] Tyrosine [15] Glutamine [10] Threonine [8] alpha-Ketoglutarate [5]  

What are the three main components of the starting material for cellulosic biofuel? In addition, name the three main steps needed in the industrial process to convert cellulosic biomass to biofuels?

Which actions performed by the student in honor lock are subject to a zero on the test/quiz? (Some answers may be due to honor lock would prohibit the student to access the assessment)

Students utilizing honor lock or any platform (including paper) are still REQUIRED to abide by all applicable programmatic testing policies/guidelines or they could receive a zero on their exam/quiz along with receiving a special admissions program disciplinary action under the handbook’s code of conduct.  

Which actions performed by the student in honor lock are subject to a zero on the test/quiz? (Some answers may be due to honor lock would prohibit the student to access the assessment)

Honor Lock can only be accessed using a NTCC issued device?

Equivalent units of production with respect to materials for ZeeZee for May, 2014 (assuming FIFO process costing is used is: