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The biochemistry lab class was given some trypsinogen that is synthesized in vitro using cell lysates containing all components necessary for transcription and translation. When they ran the enzyme activity assay, they found the enzyme does not exhibit any protease activity.  Why?

Consider the structure of ATP synthase:

In a few sentences, describe what you have learned about protein folding in this course. Questions to address in your response (address at least 3-4): How does protein folding occur? What determines the shape of a protein? How many folded shapes do most proteins form? What is the relationship between protein folding and activity? What happens to mis-folded proteins?

Control of pyruvate oxidation: The pyruvate dehydrogenase complex is regulated in more than one way. Describe two different ways (mechanisms) by which this complex can be regulated. For each one, name the type of regulation/control, describe how the regulation is mediated, and include a brief rationale.

This question is about the specificity of serine proteases such as chymotrypsin.  I want to design a mutant so that it would selectively cleave a protein at the peptide bond right after glycine.  Explain how you would alter the protein and justify your answer.  In your answer, (a) specify the part of the protein that you would modify and (b) what type of residue(s) you would put there (description is required; specific design is not). Then, justify your answer by describing how that will accomplish the desired goal.

General Chemistry Lab II – Level 2 Which of the following statements is false?

General Chemistry Lab II – Level 1 Which equation would be most helpful for determining the heat released when a metal sample is placed in a calorimeter?

Level 1 – K and ΔG Which of the following is correct regarding Gibbs Free Energy (Δ G) and Equilibira (K)?

Hess’s Law – Coffee Cup Calorimetry  – Level 1 You are performing a reaction in a coffee cup calorimeter. You add a 1.00 g piece of magnesium metal (Mg) to 100.0 mL of 1.00 M hydrochloric acid (HCl). The reaction that occurs is:Mg(s) + 2HCl(aq) → MgCl₂(aq) + H₂(g)During the reaction, the temperature of the solution increases from 25.0°C to 32.5°C. Assume the specific heat capacity of the solution is the same as water, 4.18 J/g°C, and the density of the solution is 1.00 g/mL.Calculate the enthalpy change of the reaction (ΔH) using Hess’s Law.

Which of the following gram-positive bacilli is associated with infections in neonates, pregnant women, elderly adults and outbreaks of food contamination?