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Question 3 Short Answered Questions – (20%) Below is a pyrog…

Question 3 Short Answered Questions – (20%) Below is a pyrogram generated from analysis of DNA methylation of the NFATC1 gene promoter by pyrosequencing. Four CpG sites are indicated by grey bars. 3a) What is the DNA sequence? (2 marks) 3b) Is the region methylated or unmethylated in this sample? (2 marks) 3c) Explain how the incorporation of a nucleotide during pyrosequencing is used by an enzyme cascade to produce light. (3 marks) 3d) Explain why DNA is treated with sodium bisulfite for analysis of DNA methylation. (3 marks) — ESSAY QUESTION – (80%) Explain how DNA methylation and histone modifications regulate gene expression. In your answer, discuss how epigenetic modifications can enable monoallelic gene expression.

There are FIVE questions from which you are required to answ…

There are FIVE questions from which you are required to answer only THREE; any additional answers will not be marked. All questions are comprised of an essay (80%) and short answered questions (20%), totalling 100% overall per question. Please refer to the steps below: Five text boxes are below this text box, one box for each question you can answer, each contains an essay question and short answered questions. Below the five question text boxes are 3 answer boxes where you will be answering the questions you wish to answer. Remember, you only need to answer THREE of the five available questions, which is why you have just 3 answer boxes. Within each answer box, please outline at the start which question/section you are answering to, and please label which part of the question you are answering, for example: ‘ I have chosen to answer all parts of Question 1: 1a) 1b) … Essay-  My answer to the Essay Question is: ‘