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When working with RNA it’s acceptable to use ddH2O to make solutions so long as all instruments coming in contact with the product are certified nuclease-free or autoclaved.

When using organic solvents to extract RNA, the RNA fraction will be found where after centrifugation?

After weighing the masses of Tris and EDTA you calculated in the previous question, you dumped the powders in a standard lab beaker, added ddH2O to the 100mL mark on the beaker and stirred until the powder was completely dissolved. Is this solution correctly made and ready for use as described (yes or no)? Justify your answer.

You need to make 100mL of TE pH 8.0 buffer (consisting of 120mM Tris and 1mM EDTA) for a DNA extraction. You have Tris base powder and EDTA powder from which to make it. Tris powder has a MW of 121g/mol, while EDTA powder has a MW of 292g/ml. [Answer 1] How much Tris powder will you need for this desired volume of TE solution? [Answer 2] How much EDTA powder will you need?

To make 1 liter of 5X SSC, mix together [answer 1] ml of 20X SSC and [answer2] ml of ddH2O

How much salt (NaCl) do you need to make 200mL of a 4M solution? NaCl has a molecular weight of 58.44 g/mol. Your answer should reflect the accuracy and precision of the measuring device.

What is the purpose of diethylpyrocarbonate (DEPC) in nucleic acid extraction protocols?

What property of trypan blue and similar liquids contributes to errors or inaccuracies in measurements, especially when pipetting? In other words, what led to your obtaining pipetting CV values greater than 10% during your skills assessment (assuming, of course, you weren’t yet a pipet champ)?

What is the purpose of loading dye in agarose gel electrophoresis?

An apartment complex with 342 apartments is being built. Each workday, a heating and air-conditioning system can be installed in 9 apartments. How many workdays will it take to install all of the systems? [BLANK-1]