Develop an Indirect ELISA to assess COVID-19 immunity in pat…

Develop an Indirect ELISA to assess COVID-19 immunity in patient serum samples.    Outline the sequential arrangement of reagents and samples in the wells. Specify what components are laboratory reagent(s) and what the patient sample contains when positive. Be specific. Don’t just state ‘antigen’, what is the antigen?  Don’t just state ‘primary antibody’.  What is the antibody directed against?  What class of antibody are you trying to detect? You can assume that all appropriate wash buffers are provided and do not need to specify when those would be used–this will be a heterogeneous immunoassay. I have included a picture of the COVID-19 virus structure if needed.  Must provide/include detailed descriptions of each component placed in the wells, what would you place in the wells first, second, third, fourth?  Be specific.   1. 2.  3.  4. 

You want to design a Randomized Control Trial to evaluate th…

You want to design a Randomized Control Trial to evaluate the treatment effectiveness of VMO training during closed kinetic chain exercises for patients with patella-femoral pain. Three groups will be compared: a control group (no treatment), a group with general exercise programs and a group with a specific program designed based on closed kinetic chain exercises for VMO.  Although as part of the sampling process a computer randomization has been planned, you are afraid that the variables of gender and previous physical activity might alter your results. What statistical analysis will help to control for the presence of this confounding variable?  

A physical therapist wants to know if stress management tech…

A physical therapist wants to know if stress management techniques produce an improvement in pain management for patients with chronic neck pain. A quasi-experimental study design that compares a control group vs intervention group was completed. The intervention consisted of a one-month stress management program. The outcome variables of pain intensity and Neck Disability Index (NDI) (both measured as continuous variables) were collected at the end of treatment, 3-months and 6 months follow up.    After analyzing for normality, both the variables of pain and disability were found to be normally distributed. What statistical test do you expect was used to compare the homogeneity of both groups at baseline only?  

The average cost (COST) of heating a home is a function of o…

The average cost (COST) of heating a home is a function of outside Temperature (TEMP), thickness of Insulation (INSUL) and Age of furnace (AGE). Data is collected on these variables and a regression analysis is done on the data. An incomplete MS Excel output is shown below.   Regression Statistics         Multiple R           R Square           Adjusted R Square           Standard Error           Observations                        ANOVA             df SS MS F Signifcance F Regression   171220       Residual           Total 19 212916                     Coefficients Standard Error t Stat P-Value Lower 95% Intercept 427 59.6 7.17     TEMPT (X1)   0.7723 -5.93     INSUL (X2) -14.8 4.754       AGE (X3) 12.1 4.012         The value of R-Square is: 

Exam Integrity Instructions: Before starting the exam, hold…

Exam Integrity Instructions: Before starting the exam, hold up your cell phone and tablet (if applicable) to the camera, then place them out of reach behind you. If you are wearing a smartwatch, put it on airplane mode or remove it for the duration of the exam. If using scratch paper, show both sides to the camera to confirm they are blank before beginning. **Failure to follow these instructions may result in a loss of points or a score of zero on the exam** True or false question: I have performed these tasks on camera

PART I: ESSAYRecommended: 1 Hour. 40% of final exam gradeAmy…

PART I: ESSAYRecommended: 1 Hour. 40% of final exam gradeAmy Attorney was just admitted in December 2021 and has decided to open her own practice focusing on personal injury.  She starts an Instagram account in December 2021 calling herself the PI Expert specializing in personal injury.  As soon as she opens her practice Amy makes a deal with Chester, a chiropractor, who agrees to pass along Amy’s name to his patients who need treatment after an auto accident or slip and fall. When Amy settles any lawsuit or claim for Chester’s patients, she agrees to send a gift equal to 5% of the total attorney fee to Chester when Amy recovers on cases referred by Chester. Amy also agrees to send any clients needing treatment to Chester, and Chester will give Amy a gift of 10% of the total billing recovered upon settlement.  Chester becomes the “marketing” director for Amy’s firm and manages Amy’s Instagram and other social media as part of the agreement.Penelope finds Amy on Instagram and comes in for a consultation after an auto accident Penelope says was caused by David Driver. Penelope has back problems which were exacerbated by the accident. Amy verbally offers Penelope a contingency agreement where Amy is to recover 1/3 of the total recovery.  Amy sent a written agreement to Penelope, but it is never signed.  Amy has an attraction to Penelope, which is returned.  They start a sexual relationship to their mutual satisfaction.On Amy and Penelope’s six month anniversary, Otto, David’s defense attorney (and Amy’s opposing counsel) observed one of the couple’s intimate dinners by accident, and calls Amy one day and accused Amy of having a sexual relationship with Penelope he was going to report to the State Bar, unless she dropped the case.  Amy refused.Amy then discovers that David only had a minimum policy of $15,000/$30,000, so she tells Penelope she has to settle for the policy limits, even though Chester’s bill is over $10,000.  Left with no choice, Penelope agrees, and as soon as the settlement check is received, Amy deposits it, pays herself $5,000 (1/3 of the settlement) and then tells Penelope since the chiropractor bill is over $10,000, she will only get $1,000, because Amy was able to get Chester to reduce the lien to $9,000. Penelope did not agree to the distribution.  Amy sent a check for $9,000 to Chester and $1,000 to Penelope.  And from Chester’s $9,000, she told Chester to send her a separate check for 10% of the total chiropractor bill or $900.  Chester refuses.1. What ethical violations, if any, has Amy committed with respect to her:a. Financial arrangement with Chester? Discuss.b. Partnership with Chester? Discuss.c. Relationship with Penelope? Discuss.d. Accepting Penelope’s case on a contingency basis? Discuss.2. Using the Instagram account calling herself the PI Expert.3. What ethical violations, if any, has Otto committed? Discuss.Answer according to California and ABA authorities.