Adolescent rats and humans that experience intermittent alco…

Adolescent rats and humans that experience intermittent alcohol exposure are at greater risk of developing anxiety later in life. Scientists injected viruses carrying CRISPR components into the amygdalas of mice that had NOT experienced intermittent ethanol exposure during adolescence in order to edit the mice genomes in a way that mimicked intermittent ethanol exposure.  A. What CRISPR components were the virus carrying? [dcas9KRAB]. B. How did this injection affect the Arc enhancer region? [methylation] C. How did this injection affect Arc expression? [decreased] D. How did this injection affect mice behavior? [increased]  

Dr. Wassarman also has data showing that TBI stimulates the…

Dr. Wassarman also has data showing that TBI stimulates the formation of lipid droplets that are associated with mitochondria.   Dr. Wassarman’s current hypothesis is that lipid droplet formation and trafficking to mitochondria protects neurons (at least partially) against traumatic brain injury. Which genotype would you expect to have the fewest lipid droplets near mitochondria after traumatic brain injury: A. Lis-1mutant/Lis-1wild-type B. Lis-1mutant/Lis-1mutant C. Lis-1wild-type/Lis-1wild-type  

Dr. Wassarman conducted experiments to examine DNA content i…

Dr. Wassarman conducted experiments to examine DNA content in his fly model of Ataxia Telangiectasia. Consider his Fluorescence Activated Cell Sorting (FACS) data below. First, just compare Panels A and B. In this comparison, Panel B indicates that when Ataxia Telangiectasia Mutated (ATM) is not present at wild-type (control) levels [replicate]. Next consider all the panels, and then compare Panels B and D. The best conclusion that can be drawn from this comparison is that when ATM is not present at wild-type levels [apoptosis].