Researchers conducted Northern blotting on a sample of cells…

Researchers conducted Northern blotting on a sample of cells from mouse kidney, brain, and liver using a probe that is complementary to a gene that encodes a glucose transporter.  All of the samples were taken from the same mouse. The following results were obtained: If we assume that equal amounts of total cellular RNA was loaded into each lane, which of the following statements is false?  

A primer used in dideoxy DNA sequencing is 20 nucleotides lo…

A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence:  5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to the dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown bold and underlined.    3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACTCTTCAGA-5’   Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyG had been added to the sequencing reaction?

A primer used in dideoxy DNA sequencing is 20 nucleotides lo…

A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence:  5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown in bold and underlined.  3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5’   Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyT had been added to the sequencing reaction?

The restriction enzymes shown below cut DNA in the following…

The restriction enzymes shown below cut DNA in the following manner. The slashes indicate where cutting occurs. BamHI                         G\GATCC                                     CCTAG\G   EcoRI                          G\AATTC                                     CTTAA\G        HaeII                           GGCGC\C                                    C\CGCGG   PstI                             CTGCA\G                                    G\ACGTC These enzymes will generate sticky ends that will bind to complementary sticky ends? Rank them from those that would produce the most stable sticky ends to the least stable.