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The plasmid pAEC-26 (FIGURE 1) was digested with the restriction enzymes below: A) XbaI B) BseRI + XbaI C) BseRI+ PmeI D) XbaI + PmeI After the digestion, all samples were individually loaded in a 1.5% agarose gel and the fragments were separated via gel electrophoresis. In that gel (FIGURE 2) a molecular weight marker was used in the lane 1. The problem is that the mixtures were not loaded in the order above. Can you please indicate the lines in which each of the mixtures were loaded?

After growing the transformed DH5 alpha cells in LB + sucrose plate, we then use the colonies present in this media for the second plasmid extraction. Why we don’t digest the plasmid again in this step?

All expression plasmids used in biotechnology are encoding for the SacB gene as a counter-selection marker.

The pCrazy45 was used to clone the alpha amylase gene. The plasmid map carrying the alpha amylase encoding the ORF correctly inserted downstream of the T7P is shown in the picture below. Describe the results you expect to have in agar plates having just LB sucrose 5% to recover the Escherichia coli colonies after transformation? The red circle with a line represents the T7 terminator.

Acetylcholine is a neurotransmitter that moves from a motor end plate to the sarcolemma of a muscle fiber through the synaptic cleft.  Such movement exemplifies:

The following exemplifies normal bone growth and development based on Wolf’s law except:

Globally, what region has the highest increasing prevalence  of diabetes globally?

Globally, approximately ____ in ______  adults have diabetes. 

Final Exam Prompt: For your final exam, you will write to demonstrate your ability to summarize academic articles, synthesize information from multiple sources, use MLA documentation, organize your ideas clearly, and maintain an academic tone. Prompt:Read the following two articles about the impact of social media on the mental health of teenagers and young adults. Your task is twofold: First Paragraph: Summarize the main arguments and key findings of each article in your own words, making sure to attribute ideas clearly and use MLA in-text citations. Stay in an academic tone. Second Paragraph: Synthesize the information from both articles to develop your own argument about the effects of social media on young people’s mental health. Discuss how the articles agree, differ, or complicate each other, and explain how your own position draws on their ideas. You must use both PARAPHRASING AND QUOTING with parenthetical in-text citations in MLA format. This is not a personal experience or opinion piece; therefore, do not draw on your own experiences or beliefs. Articles for Support: (click the links provided with the citations) Stoney Brooks, Does personal social media usage affect efficiency and well-being?, Computers in Human Behavior, Volume 46, 2015, Pages 26-37, ISSN 0747-5632, https://doi.org/10.1016/j.chb.2014.12.053.Twenge, Jean M. “Have Smartphones Destroyed a Generation?” The Atlantic, Sept. 2017, https://www.theatlantic.com/magazine/archive/2017/09/has-the-smartphone-destroyed-a-generation/534198/. Bandekar, Bhairavi, et al. “Social Media: Effect, Affect, Teens, and Possible Addiction.” Digital Commons Collin, digitalcommons.collin.edu/ccuisrc/2018/thursday/2/. click “download” on the page to access pdf. Requirements: Summarize both articles accurately and concisely, using MLA in-text citations. Synthesize the information from both sources to present your own clear, arguable thesis. Organize your essay with an introduction, body paragraphs, and conclusion. Include a Works Cited page in proper MLA format. Maintain a formal, academic tone throughout your essay. Standard academic paragraphs are eight or more sentences (approx. 250 words each) You will not be able to use double-space in the textbox. 

Skeletal muscle contracts voluntarily in contrast to cardiac and smooth muscles.  Voluntary contraction means: