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When using a cryostat for muscle biopsies, what would be the expected outcome of the stained section if  the tissues were allowed to slowly freeze?   

Identify the corrective action that would be taken here. 5.jpg 

Which fixative is useful for a post fixative of electron microscopy as it will preserve phospholipids?

When observing the results of a Von Kossa stain microscopically, it is noted that the calcium salts are staining a yellow to yellow brown color instead of the expected black. What is a plausible explanation for these results?

A pathologist requests both the Alcian blue, pH 2.5 and a Periodic Acid Schiff stain to be done on a tissue sample. These stains can be used to differentiate:

Formalin pigment is identified in a section of spleen. This pigmentation can be removed by treating the sections with:

A pathologist suspects that a patient has Alzheimer’s disease, what stain would be helpful in making this determination?

A quality control record indicates the paraffin melting point is between 52 C to 54 C degrees. Ideally the temperature for the tissue flotation bath should be established at:

A section of skin shows an abundant amount of melanin which is obscurring the cellular detail.  Which technique can be used to bleach melanin from tissues?

Microscopic evaluation of a Masson trichrome reveals that the connective tissue is staining a faded blue instead of the expected crisp bright blue color. What can be done to prevent this from happening again?