Your goal is to optimize a tissue culture protocol for the p…

Your goal is to optimize a tissue culture protocol for the production of embryogenic calli with high plant regeneration potential from a new potential biofuel crop: A. (8) List 4 factors beside culture medium components and aseptic conditions which will likely determine the tissue culture response. B. (2) Explain what is totipoteny in tissue culture. C. (2)  How do you to direct the culture response to undifferentiated (callus) or differentiated (plant regeneration) growth. D. (4) Describe two approaches/types of treatments that can minimize the occurrence of genetic variation in tissue culture?  E. (12) Describe 8 steps/treatments that prevent contaminations in tissue cultures.

After a genome editing tool has created a DNA double strand…

After a genome editing tool has created a DNA double strand break in the coding sequence of a specific gene (e.g. lignin biosynthetic gene) in the plant genome the cell needs to repair this break. Describe two different repair outcomes and the consequences for the expression and function of this specific gene.