Author: Anonymous

Peak 2 below from an ion exchange column shows

A pregnancy test is equivalent to which of the following techniques?

Which technique involves concentrating proteins by placing the sample in special device that puts nitrogen pressure on the sample to force the buffer through a membrane?

You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

A protein has a pI of 5, and is in a buffer at pH 7. What type of column should it bind to?

Which of the following would be best to study the glycosylation state of a protein?

You use bacterial two-hybrid to study proteins A and B below. If you randomly mutate protein A at the position noted,

Mass spectrometry can be used to determine all of the following EXCEPT

Hemoglobin contains 4 subunits (2 α, 2 β) of 141 and 146 amino acids, respectively. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column, and you only see peaks in fractions 30 and 40. What would you conclude?