You are interested in performing blue and white screening. You plate cells containing a vector with no insert in the presence of ampicillin and X-gal (no IPTG). Which of the following would you expect to observe?
Author: Anonymous
To verify the sequence of an insert in a vector with Sanger…
To verify the sequence of an insert in a vector with Sanger sequencing, you must use
In ligation independent cloning,
In ligation independent cloning,
You perform Sanger sequencing on a plasmid which came from a…
You perform Sanger sequencing on a plasmid which came from an E. coli colony. At one position you find two different overlapping peaks at 50% of the height of surrounding peaks, which are all identical to one another. What can you conclude from this?
What primers would you design to amplify the following seque…
What primers would you design to amplify the following sequence of DNA? 5’-TTGCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTAGAGTC-3’
100 micrograms/ml of DNA would give an A260 of
100 micrograms/ml of DNA would give an A260 of
In the gel below, the DNA in Lane 4 is approximately
In the gel below, the DNA in Lane 4 is approximately
You use a dot blot to analyze a specific locus. Who is homoz…
You use a dot blot to analyze a specific locus. Who is homozygous at this locus?
What is the advantage to introducing mutations into gene tha…
What is the advantage to introducing mutations into gene that is in already in a vector, rather than from a piece of linear DNA?
VNTR analyzes differences in DNA between individuals by comp…
VNTR analyzes differences in DNA between individuals by comparing