Author: Anonymous

Why aren’t most proteins purified with organic solvents?

When would a researcher use chaotropic salts in a purification?

You purify a mouse his-tagged protein and analyze it on SDS-PAGE and western blotting. What is TRUE about primary antibodies against this protein?

You plan on purifying a protein over two columns. Between which types of chromatography should you perform dialysis to ensure the second column runs correctly?

You wish to express a protein expression system that is very fast, inexpensive, and has established protocols that have been perfected over the past few decades, rather than explore a newer system. Which expression system would you choose?

Peak 2 below from an ion exchange column shows

A pregnancy test is equivalent to which of the following techniques?

Which technique involves concentrating proteins by placing the sample in special device that puts nitrogen pressure on the sample to force the buffer through a membrane?

You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

A protein has a pI of 5, and is in a buffer at pH 7. What type of column should it bind to?