Author: Anonymous

Which is TRUE about N-terminal sequencing?

Proteins can be concentrated using all of the following EXCEPT

You purify 100 ml of protein, and the buffer has 100 mM NaCl. If you perform dialysis in a 1 L beaker of buffer with no salt overnight, the final salt concentration will be approximately

A protein that can be acetylated has five domains (A through E). Researchers deleted these domains in the western blot of an SDS-PAGE below. From this data, you can conclude that

What is the molecular weight of the band found in Lane 3 below?

Organic chemists can synthesize polypeptides. Why aren’t most proteins made this way?

A technique that is useful in trying to identify new protein-protein interactions from a homogenate is

Which of the following is an advantage of in vitro protein expression?

Talin (257 kDa) binds to actin (43 kDa). You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. Which band represents the protein of interest?