Author: Anonymous

Which of the following is an advantage of in vitro protein expression?

Talin (257 kDa) binds to actin (43 kDa). You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. Which band represents the protein of interest?

The beads in a cation exchanger are

When running size exclusion chromatography, you get two peaks merged together as seen below. Which of the following is the most appropriate solution for separating the two peaks?

A his-tagged protein can be purified using all of the following techniques EXCEPT

The image below, which illustrates an increased size on the gel after the addition of Protein 2, is demonstrating

Which of the following is a disadvantage of in vitro protein expression?

Which of the following window elements displays the left and right document margins?

Sarah wants to apply a set of effects such as shading, borders, and shadows to a picture. Which of the following can she use to do this?