Author: Anonymous

A VNTR analysis is performed on a mother, father, and child at a specific locus, shown below. Which of the following is TRUE?

You want to clone a 5000 bp piece of DNA. What is the best way to accomplish this?

DNA can be detected in an agarose gel by using each of the following EXCEPT

You insert a gene into the vector below using the SacI and AvrII cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

You insert a gene into the vector below using the HpaII and EcoRI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

Which of the following techniques involved TA cloning to sequence the human genome in fragments?

If you digested the vector below with TseI and HpaI, how many bands would you see on a gel?

T4 DNA polymerase and dGTP are mixed with the dsDNA below. Which of the following is TRUE? 5’-ACCTGTAGCTG… 3’-TGGACATCGAC…

Why does linear DNA separate based on size in an agarose gel?

In qPCR, SYBR Green is not specific to the gene of interest. What is used to account for this?