Author: Anonymous

A mutation (GCC) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGGCGCCGCGACAGATTTGAC-3’

What technique involves using a terminal deoxynucleotidyl transferase to add multiple Gs to the end of an unknown DNA sequence for amplification?

Which technique allows one to screen bacterial strains for mutations, or to track down the origin of a pathogen in food?

Which of the following is FALSE regarding Taq and Pfu DNA polymerases?

The image below is demonstrating

Which technique would be best for single cell DNA sequencing?

All of the following were essential to the development of PCR EXCEPT

To introduce a His-tag onto a protein at the C-terminus, you would need to

You design two primers with melting temperatures of 50 °C and 60 °C. If both of these primers were to be used together in PCR, what should the ideal annealing temperature be?

The gel below analyzes a family via RFLP. Which of the following children are recombinant at the A locus based on the gel?