Author: Anonymous

You purify 100 ml of protein, and the buffer has 200 mM NaCl. How many dialyses will you need to perform to reduce the salt concentration to below 10 mM, if dialyzing into 1 L of buffer with no salt? 

Which technique allows one to purify proteins directly from organs (such as from gizzards)?

A protein has a pI of 7, and is in a buffer at pH 5. What type of column should it bind to?

The native gel below shows three proteins (A-C) run alone, and then with a protein of interest (P). Which protein interacts with protein P?

In which expression system does T7 RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?

TARP protein (with domains A through D) was tested to determine its ability to polymerize actin, which will then be found in the pellet after centrifugation. Which of the following is TRUE regarding TARP?

The gel below of an E. coli induction with IPTG is showing

What pH must you use to purify a protein with a pI of 4.2 via anion exchange chromatography?

When the phage bacterial expression system is off,

Why can’t SDS-PAGE be used as a purification technique for large amounts of protein?