Author: Anonymous

You wish to express a protein expression system that is very fast, inexpensive, and has established protocols that have been perfected over the past few decades, rather than explore a newer system. Which expression system would you choose?

Peak 2 below from an ion exchange column shows

A pregnancy test is equivalent to which of the following techniques?

Which technique involves concentrating proteins by placing the sample in special device that puts nitrogen pressure on the sample to force the buffer through a membrane?

You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

A protein has a pI of 5, and is in a buffer at pH 7. What type of column should it bind to?

Which of the following would be best to study the glycosylation state of a protein?

You use bacterial two-hybrid to study proteins A and B below. If you randomly mutate protein A at the position noted,

Mass spectrometry can be used to determine all of the following EXCEPT

Hemoglobin contains 4 subunits (2 α, 2 β) of 141 and 146 amino acids, respectively. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.