Author: Anonymous

If a codon for an amino acid is 5′ AGC 3′ then what is the anticodon that would recognize this codon? For your answer write ONLY the sequence of the codon in the 5′ to 3′ direction. Do not add the 5′ or 3′ numbers and do not use any other punctuation. Your answer should look like: GGG

If the following piece of double-stranded DNA is transcribed, using the TOP strand as the template strand, what would be the RNA molecule produced?   5’ACCGTGACACGT3′ 3’TGGCACTGTGCA5′

During translation, three sites in the ribosome are formed that house tRNA molecules in different stages of the translation process. During initiation, before a peptide bond has been made, the E site contains [answer1], the P site contains [answer2] and the A site is the location for [answer3]. 

True/False regarding bacterial transcription. A strong promoter has a promoter sequence that is close to the ideal sequence for the sigma factor that recognizes that promoter.

Finish the following sentence. This is a multiple answer question.   Mutations may occur through …

True/False. Another type of post-transcriptional regulation is antisense RNA. In this case, the antisense RNA binds to the mRNA to exert its regulatory effect.

You are studying a new operon in a bacterial species.  The rib operon codes for genes needed for the use of ribitol as a carbon and energy source. The rib operon is subject to both positive and negative transcriptional regulation, at the level of initiation. An activator protein called RibG binds the rib operator and promotes transcription when ribitol is available. When ribitol is not present, the RibG protein no longer does this. So, when ribitol is present you would expect that transcription would be [blank1] and when ribitol is absent you would expect that transcription would be [blank2].

True/False. One way for a mutation to happen is if RNA polymerase makes a mistake during the transcription process.

True/False. One example of post-transcriptional regulation is the riboswitch method. This is when the binding of a small molecule affects mRNA structure.  

You are studying a gene and identify a mutation in the coding sequence that results in a UUC codon (phenylalanine) in the mRNA being changed to a UUA codon (leucine). What kind of mutation is this and what can you tell about the impact of this mutation on the function of the protein coded for by this gene?