Imagine you have engineered a mutant transposase where the c…

Imagine you have engineered a mutant transposase where the conserved E of the DDE motif is mutated to alanine (A). The N-terminal DNA-binding domain of the protein remains perfectly intact. If you were to perform an in vitro transposition assay with this mutant, what specific outcome would you observe?

A 12-year-old with known Type 1 DM presents with glucose of…

A 12-year-old with known Type 1 DM presents with glucose of 490 mg/dL, pH 7.17, bicarbonate of 8 mEq/L, and moderate ketonuria. He is tachycardic with a capillary refill of 3 seconds and no hemodynamic instability. What is the CORRECT initial fluid and insulin management according to current DKA guidelines?