As shown at left in the diagram below, a plasmid vector has…

As shоwn аt left in the diаgrаm belоw, a plasmid vectоr has three Eco RI sites. The vector also has one Bam HI site located within the polylinker (Multiple Cloning Site). The numbers in kb (1 kb = 1000 bp) are the numbers of base pairs between two adjacent EcoRI sites. After digesting this vector to completion with either Eco RI or Bam HI in separate reactions, you electrophorese each reaction in separate lanes on an agarose gel. On the right is the result of gel electrophoresis produced by exposing the ethidium bromide–stained gel to UV light. What are the sizes of DNA fragments in the four bands from top to bottom?