Cancer 1 You are a human geneticist studying cancer. You hav…
Questions
Cаncer 1 Yоu аre а human geneticist studying cancer. Yоu have fоur cell types (Q, R, S, T) that have been derived from four different tumors (each from a different patient with a different type of cancer). You have designed a PCR-based assay to detect large chromosomal abnormalities such as deletions, duplications, inversions, and translocations. It turns out that each of your cell types has a different one of these abnormalities affecting either one or both of the following chromosomal regions (Regions 1 & 2). In each cell type, a different chromosomal abnormality contributes to the development of the cancer in these cells. In the diagram below, the small arrows indicate PCR primers you will be using in your assay. Note that Regions 1 and 2 are not the same size (i.e. they are not drawn to scale in the drawing). You do PCR using four different pairs of primers (in four separate reactions) on each of the four cell lines, and wild-type cells. You run the PCR products on a gel, a schematic of which is shown below. The heavier bands indicate more DNA. The primers used are listed at the top of each lane in the gel. For each cell type, choose the type of rearrangement, and whether the individual is homozygous or heterozygous for the indicated chromosomal abnormality. If you cannot tell, choose “inconclusive” Cell Type T: Cell Type Q: Cell Type R: Cell Type S:
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