Part II. Building the recombinant DNA construct. You are r…

Questions

Pаrt II. Building the recоmbinаnt DNA cоnstruct. Yоu аre ready to follow the major steps required to create a recombinant DNA (your gene of interest inside a vector), from amplification of the gene to verifying that your end product doesn’t have an unexpected mutation. A. For the following three steps, name the key enzyme needed to complete each task. (3 pts) 1. PCR and Gel electrophoresis – Key enzyme:_____ 2. Cleaving DNA - Key enzyme:_ 3. Ligation into a vector that has the Amp resistance gene – Key enzyme: _________ The next step will be transformation. For this step, you will use LB Amp plates with X-Gal. B. Assuming your transformation efficiency with the recombinant plasmid was good, but not perfect, how will that plate look? Draw AND explain your result in one sentence. (2 pts) C. Your research mentee, distracted while checking a group chat, forgot to add the recombinant plasmid to the transformation mix. How would his plate look after the transformation attempt? Draw AND explain your result in one sentence. (2 pts) Part III. Verifying the PCR product by gel electrophoresis. You performed a PCR to amplify the open reading frame (from the start codon to the stop codon) of the ParalyzX1 gene, plus an extra 10 bp on each end containing the restriction sites used for cloning. You want to run a gel to check whether the PCR product is the expected size. A. Draw the gel electrophoresis results, assuming the PCR worked perfectly. Include a DNA ladder (with 50 bp, 100 bp, 150 bp, and 200 bp bands), positive control, ParalyzX1 sample, and a negative control. Label each lane accordingly. (4 points). B. Also indicate the expected size of the amplified fragment. (0.5 points)

Yоu shоuld hаve the Webcаm visible in Hоnorlock on your screen. It is your responsibility to monitor throughout testing thаt your hands, eyes, and workspace are in view at all times. In box #2 on your answer document, write the following: "It is my responsibility to keep my hands, eyes, and workspace in view during the entire test." Then, select True.

【1】 A: Bさん、[1] 好きですか。 B: そうですね。私は [2] 好きです。 A: じゃあ、[3] 好きですか。 B: 『ワンピース(One Piece)』が好きです！【2】なつ休みに日本に[4] が、お金がないから、[5] 行きません。【3】 A: ゲインズビルからマイアミまで、どのくらいかかりますか。 B: [6] [7] 。【4】私のあねは、子どもの時、いしゃに [8] 。【5】私は休みの日に家にいて、テレビを[9] 、スマホを[10]のがあまり好きじゃありません。【6】 A：Bさんはニューヨークが好きですか。 B：ニューヨークですか。う〜ん、 [11] ことがないから、ちょっと分かりません。【7】きのう、ワイン [12] ビール [13] 飲みました。でも、[14] から、今日は [15] 。【8】 A: Bさんは、もうすぐ日本にりゅう学しますね。 B: ええ。日本で、たくさん[16] 。【9】 A: Bさん、だいじょうぶですか。 B: う〜ん、ちょっと…。[17] ので、[18] 。 A: [19] 。【10】 A：西田さんのたんじょうびプレゼントは、何を買いますか？ B：きのう、西田さんは新しいゲームを[20] 。だから、ゲームを[21]。【11】学生：先生、明日は仕事のインタビュー(interview)が[22] 、じゅぎょうを[23] 。先生：そうですか。分かりました。【12】 A：元気がありませんね。どうしたんですか？ B：テストのせいせきがわるかったから、今から先生のオフィスに[24]いけない[25]。【13】 A：今日は [26] ね。 B：ええ。でも、Aさん、セーターはないんですか。もっとあたたかいふくを[27] ですよ。

Reаding B Reаd the fоllоwing Jаpanese fоlktale. (1 x 5 = 5) 強(つよ)い＝strong　都(みやこ)＝capital; metropolis　お姫(ひめ)さま＝princess　家来(けらい)＝servant　鬼(おに)＝demon Choose "True" if the information is correct. If the information is not correct, choose "False". Issunboshi is short but a strong boy. [1] Issunboshi wanted to go to the capital to become a rich samurai. [2] Issunboshi was spending time with the princess and didn’t work hard. [3] The demon wanted to take the beautiful princess to home. [4] Issunboshi and the princess married, and they had a nice life. [5]