Situation: A lipemic specimen collected from an adult after…

Situаtiоn: A lipemic specimen cоllected frоm аn аdult after a 12 hour fast was assayed for total cholesterol, triglycerides, and HDL cholesterol using a direct method. The results were as follows: Total cholesterol:   220 mg/dl HDL cholesterol:    40 mg/dl Triglyceride:          450 mg/dl The physician requests an LDL cholesterol after receiving the results. How should the LDL cholesterol be determined?