Gel electrophoresis is a laboratory technique used to separa…
Questions
Gel electrоphоresis is а lаbоrаtory technique used to separate DNA fragments based on size. This method is especially useful in analyzing DNA obtained from techniques like polymerase chain reaction (PCR) or DNA fingerprinting. The process involves placing DNA samples into wells within a gel, typically made of agarose, and applying an electric current. DNA molecules, being negatively charged due to their phosphate backbone, migrate toward the positively charged electrode.The movement of DNA fragments through the gel matrix is size-dependent: smaller fragments encounter less resistance and thus travel farther, while larger fragments move more slowly and cover shorter distances. This separation allows for the visualization of DNA fragment sizes when stained with a dye and illuminated under UV light.In DNA fingerprinting, gel electrophoresis can reveal patterns of short tandem repeat (STR) fragments, which vary between individuals. This makes it a powerful tool for applications such as paternity testing and forensic investigations, where matching DNA patterns is crucial. For example, in paternity testing, a child’s DNA pattern is compared with the potential parents. The child’s fragments must match a combination of those inherited from both the mother and the father, as shown in the provided gel image.A child’s DNA pattern obtained through gel electrophoresis is being analyzed to determine paternity. The child’s STR bands match both the mother’s pattern and additional bands from one of two potential fathers. What is the most likely conclusion?
During аdmissiоn tо а skilled nursing fаcility, an оlder adult presents with multiple medications, including warfarin, low-dose aspirin, Ginkgo biloba, and echinacea. Which potential interaction should the nurse bring to the provider’s attention?