9. OPTICAL TRAPS  To further understand interactions between…

Questions

9. OPTICAL TRAPS  Tо further understаnd interаctiоns between HERKY3’s dоwnstreаm targets, you useoptical traps to measure the interaction forces between two proteins. a. (1 pt) In your calibration measurements you find that the bead stiffness is toohigh and that you are losing sensitivity. What is one way in which the beadstiffness can be reduced so that your measurements become more sensitive? b. (1 pt) True/False. Optical trapping is only appropriate on subcellular scales (i.e.,purified proteins and structures). c. (3 pts) Which force measurement mode is most appropriate to characterizemovement of a translocating molecule, such as a molecular motor? Brieflyexplain the principle of this mode.

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