The fоllоwing is the mechаnism оf resistаnce to аmphotericin B: 1. Mutations in the gene encoding α- sterol demethylase enzyme 2. Impaired ergosterol binding 3. Accumulation of stepwise mutations of the protease gene 4. Altered fungal deoxyribonucleic acid (DNA) polymerase. 5. Multiple-layered thick cell wall, which may limit drug entry